Human BAI1 Antibody (542515)

R&D Systems | Catalog # MAB4969

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 542515
View all BAI1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BAI1
Met1-Thr879
Accession # O14514

Specificity

Detects human BAI1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BAI3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BAI1 Antibody (542515)

Detection of Human BAI1 by Immunohistochemistry

Detection of Human BAI1 by Immunohistochemistry

Localization of BAI1, Noggin, Iba1, NeuN and GFAP in the mouse brain.Tissue sections from the day-10 mouse brain were stained with hematoxylin and eosin (sagittal sections A and D; coronal sections B and C) or double labeled with the G8 and the R&D BAI1 mAbs, or G8 and antibodies to Iba1, NeuN or GFAP. The areas within the boxes of the H&E stained sections are shown at high magnification in the fluorescence photomicrographs. The colors of the fluorescent secondary antibodies are indicated in the unmerged photographs (E, F, H and I). Nuclei were stained with Hoechst dye. Overlap of red and green, when present, appears yellow in merged images (G, J, K, L and M). The G8 and BAI1 mAbs labeled the same subpopulation of cells in the hippocampal formation (box in A; E-G). The Noggin and BAI1 antibodies also bound to the same cells in the glomerular layer of the olfactory bulb (box in B; H-J). G8 did not co-localize with Iba1 (K, from box in A), NeuN (L from lower box in C) or GFAP (M from box in D). Minimal fluorescence was observed with the anti-IgM and anti-IgG (inset in G) or the anti-goat and anti-IgG (inset in J) secondary antibodies. Bar = 270 μM in A-D and 9 μM in E-M. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32614850), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human BAI1 Antibody (542515)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alpha V beta 5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

  1. Nishimori, H. et al. (1997) Oncogene 15:2145.
  2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
  3. Mori, K. et al. (2002) Neurosci. Res. 43:69.
  4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
  5. Park, D. et al. (2007) Nature 450:430.
  6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
  7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
  8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
  9. Kaur, B. et al. (2005) Oncogene 24:3632.
  10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
  11. Kaur, B. et al. (2009) Cancer Res. 69:1212.
  12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
  13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 Antibody (542515)

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Product Specific Notices for Human BAI1 Antibody (542515)

For research use only

Citations for Human BAI1 Antibody (542515)

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