Human BRIP1/FANCJ Antibody

Catalog #: MAB6496 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6496
MAB6496-SP

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Detection of Human BRIP1/FANCJ by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BRIP1/FANCJ Antibody Summary

Species Reactivity

Human

Specificity

Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.

Source

Monoclonal Mouse IgG₁ Clone # 652747

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BRIP1/FANCJ
Ser2-Arg160
Accession # Q9BX63

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BRIP1/FANCJ by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRIP1/FANCJ at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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BRIP1/FANCJ in Human Breast Cancer Tissue. BRIP1/FANCJ was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRIP1/FANCJ

BRCA1-interacting protein 1 (BRIP1), also known as Fanconi anemia complementation group J protein (FANCJ) and BACH1, is a 130 kDa ubiquitously expressed DNA helicase. BRIP1 binds to the BRCT domain of BRCA1 and regulates BRCA1 dependent DNA repair and cell cycle checkpoint control. It is mutated in patients with Fanconi anemia, a disorder characterized by chromosomal instability and increased risk of developing cancer. Within amino acids 1-160, human BRIP1 shares 78% and 74% aa sequence identity with mouse and rat BRIP1, respectively.

Long Name

BRCA1 Interacting Protein C-terminal Helicase 1

Entrez Gene IDs

83990 (Human)

Alternate Names

ATP-dependent RNA helicase BRIP1; BACH1; BACH1BRCA1/BRCA2-associated helicase 1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRIP1; EC 3.6.1; EC 3.6.4.13; FACJ; FANCJ; FANCJFanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ