Human Cadherin-17 Antibody

Detection of Cadherin-17 by Western Blot

Postn+ cell lineage tracing during lung fibrosis.(A) Schematic representation showing the genetic strategy for the generation of the Postn-CreERT2; mT/mG mice for lineage tracing. (B) Schematic diagram of the experimental design. Postn-CreERT2; mT/mG mice were challenged to a single intratracheal inhalation of 1 U/kg BLM followed by injection with tamoxifen on 5 consecutive days. The control mice (Postn-CreERT2; mT/mG) without BLM injury (–BLM) were induced by 5 consecutive days tamoxifen and analyzed at day 21 after tamoxifen injection. (C) Representative images of Postn+ lineage GFP+ cells in Postn-CreERT2; mT/mG mice after tamoxifen treatment and BLM challenge. Immunofluorescent staining showing tdTomato and GFP single channels in addition to a merged image. Scale bar: 20 μm. (D and E) Western blot analysis (D) and quantification (E) of the indicated protein levels in the lung of mice at 21 days post bleomycin injury (d.p.i.). n = 4–8. (F) Representative images to identify the Postn+ cells in the mouse lung sections. Antibodies against the stromal markers ( alpha -SMA, Desmin, Pdgfr-beta, and Pdgfr-alpha ), endothelial marker CD31, and alveolar type 1 (AT1) cell marker Rage were costained in the lung sections of Postn-CreERT2; mT/mG mice at 21 d.p.i. Scale bar: 20 μm. (G) Quantification of colocalization of the GFP+ cells with alpha -SMA+, Desmin+, Pdgfr-beta +, Pdgfr-alpha +, CD31+, or Rage+ cells in the lung sections of Postn-CreERT2; mT/mG mice at 21 d.p.i. n = 10. (H) Representative images of Postn expression, which were costained with alpha -SMA in the lung sections of patients with IPF. Scale bar: 20 μm. n = 8. Shown are mean values ± SEM. Statistical significance was determined by unpaired Student’s t test or the Mann-Whitney U test. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.jci.org/articles/view/184158), licensed under a CC-BY license. Not internally tested by R&D Systems.