Human Calreticulin Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB38982T Datasheet / COA / SDS

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FAB38982T-100UG

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Human Calreticulin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Calreticulin in direct ELISAs. In direct ELISAs, no cross-reactivity with human Vasostatin is observed.

Source

Monoclonal Mouse IgG1 Clone # 681207

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Calreticulin
Glu18-Leu417
Accession # P27797

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Calreticulin

Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca⁺⁺-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Entrez Gene IDs

811 (Human); 12317 (Mouse); 64202 (Rat)

Alternate Names

Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; Vasostatin