Human CaM Kinase II gamma Antibody

Name: Human CaM Kinase II gamma Antibody
Brand: R&D Systems
SKU: AF7280

Catalog #: AF7280 Datasheet / COA / SDS

Newer Version Available: MAB7280

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Discontinued Product

AF7280 has been discontinued and is replaced by MAB7280.

Detection of Human CaM Kinase II gamma by Western Blot.

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Human CaM Kinase II gamma Antibody Summary

Species Reactivity

Human

Specificity

Detects human CaM Kinase II gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) CaM Kinase II beta is observed, and less than 5% cross-reactivity with rhCaM Kinase II alpha and rhCaM Kinase II δ is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CaM Kinase II gamma
Ala448-Gln558
Accession # Q13555

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CaM Kinase II gamma by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase II gamma at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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CaM Kinase II gamma in Human Brain. CaM Kinase II ? was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human CaM Kinase II ? Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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CaM Kinase II gamma in Mouse Brain. CaM Kinase II ? was detected in perfusion fixed frozen sections of mouse brain (brainstem) using Sheep Anti-Human CaM Kinase II ? Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaM Kinase II gamma

Calcium/calmodulin-dependent protein kinase type II gamma (CaMKII gamma ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. The widely expressed CaMKII gamma from human, mouse, and rat share 100% aa sequence identity within aa 448-558 of isoform 1.

Long Name

Calcium/calmodulin-dependent Protein Kinase II gamma Subunit

Entrez Gene IDs

818 (Human); 12325 (Mouse); 171140 (Rat)

Alternate Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM Kinase II gamma; CaM kinase II subunit gamma; CAMK; CAMK2G; CAMKG; CAMKGFLJ16043; CaMK-II subunit gamma; CAMK-II; EC 2.7.11; EC 2.7.11.17; MGC26678