Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280

Specificity

Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Integrin  alpha M and recombinant human Integrin  alpha E is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Canine CD11b/Integrin alpha M Antibody

Detection of CD11b/Integrin aM antibody in Canine PBMCs antibody by Flow Cytometry.

Detection of CD11b/Integrin alpha M in Canine PBMCs by Flow Cytometry.

Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine CD11b/Integrin aM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of CD11b/Integrin alpha M in PBMC monocytes cells by Flow Cytometry.

PBMC monocytes cells were stained with Goat Anti-Human/Canine CD11b/Integrin alpha M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Canine CD11b/Integrin alpha M Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells and canine peripheral blood mononuclear cells (PBMCs)

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD11b/Integrin alpha M

Reviewed Applications

Read 1 review rated 5 using AF1699 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

Human ITGAM (Integrin alpha M subunit) is a 127kDa (predicted) glycoprotein, a member of the Integrin family of proteins. The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. The ECD of human ITGAM shares 75.8% aa sequence identity with canine ITGAM ECD.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

NP_001139280

Additional CD11b/Integrin alpha M Products

Product Documents for Human/Canine CD11b/Integrin alpha M Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Canine CD11b/Integrin alpha M Antibody

For research use only

Citations for Human/Canine CD11b/Integrin alpha M Antibody

Customer Reviews for Human/Canine CD11b/Integrin alpha M Antibody (1)

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  • Human/Canine CD11b/Integrin alpha M Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: blood slides
    Species: Canine
    Verified Customer | Posted 07/25/2017
    ICC
    Human/Canine CD11b/Integrin alpha M Antibody AF1699

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