Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB11526X Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB11526X-100UG

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Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Carbonic Anhydrase IX/CA9 in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 1349A

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line, NS0-derived human Carbonic Anhydrase IX/CA9
Pro59-Asp414
Accession # Q16790

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Alternate Names

CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250