Human Caspase-1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB62153S Datasheet / COA / SDS

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FAB62153S-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspase-1 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 1021408

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

ELISA

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Caspase-1

Caspase-1, also known as IL-1 beta -Converting Enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Entrez Gene IDs

834 (Human)

Alternate Names

CASP1 nirs variant 1; CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; Caspase-1; EC 3.4.22.36; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45