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Human CCR9 APC-conjugated Antibody

Catalog #: FAB179A
2 Citations 1 Review Datasheet / COA / SDS
Clone 112509 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB179A-025
FAB179A-100

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Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry.
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Citations (2)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR9 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
Source
Monoclonal Mouse IgG2A Clone # 112509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR9 antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 APC-conjugated Monoclonal Antibody (Catalog # FAB179A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

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Citations for Human CCR9 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mucosal-Associated Invariant T Cells Display a Poor Reconstitution and Altered Phenotype after Allogeneic Hematopoietic Stem Cell Transplantation
    Authors: M Solders, T Erkers, L Gorchs, T Poiret, M Remberger, I Magalhaes, H Kaipe
    Front Immunol, 2017-12-21;8(0):1861.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Immunization of volunteers with Salmonella enterica serovar Typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vbeta repertoires.
    Authors: Salerno-Goncalves R, Wahid R, Sztein MB
    Infect. Immun., 2005-06-01;73(6):3521-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CCR9 APC-conjugated Antibody

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Human CCR9 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Cells were stained for 10 minutes in room temperature followed by three washes.
Specificity: Specific
Sensitivity: Not sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10

Flow Cytometry Human CCR9 APC‑conjugated Antibody FAB179A