Human CCR9 Fluorescein-conjugated Antibody

Catalog #: FAB179F
8 Citations Datasheet / COA / SDS

Clone 112509 was used by HLDA to establish CD designation

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FAB179F-025
FAB179F-100

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Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry.

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Product Details

Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR9 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.

Source

Monoclonal Mouse IgG_2A Clone # 112509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB179F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCR9 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
Authors: L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez
International Journal of Molecular Sciences, 2021-04-09;22(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Mucosal cell populations may contribute to peripheral immune abnormalities in HIV-infected subjects introducing cART with moderate immune-suppression
Authors: M Basilissi, C Tincati, E Merlini, G Ancona, E Borghi, F Borgo, A Barassi, A d'Arminio, G Marchetti
PLoS ONE, 2019-02-14;14(2):e0212075.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice
Authors: R Robert, C Ang, G Sun, L Juglair, EX Lim, LJ Mason, NL Payne, CC Bernard, CR Mackay
JCI Insight, 2017-08-03;2(15):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
Authors: Zaidoon Al-Jaderi
Front Immunol, 2016-10-19;7(0):437.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
J Immunol, 2014-02-19;192(6):2634-42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of human NK17/NK1 cells.
Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
PLoS ONE, 2011-10-21;6(10):e26780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CCL25 mediates migration, invasion and matrix metalloproteinase expression by breast cancer cells in a CCR9-dependent fashion.
Authors: Johnson-Holiday C, Singh R, Johnson E
Int. J. Oncol., 2011-02-23;38(5):1279-85.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology, 2004-06-01;112(2):274-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry