Human CD1c/BDCA-1 APC-conjugated Antibody

Discontinued Product

FAB5910A has been discontinued.
View all CD1c/BDCA-1 products.
Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human CD1c/BDCA-1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCA‑1
Ala19-Met302 (Phe300Ser)
Accession # P29017
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD1c/BDCA‑1 antibody in MOLT‑4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Goat Anti-Human CD1c/BDCA-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5910A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human CD1c/BDCA‑1 by Flow Cytometry View Larger

Detection of Human CD1c/BDCA‑1 by Flow Cytometry Endotoxin sensitivity correlates with CD14 expression.A Expression of TLR4 and CD14 on different human immune cells. Cells were plated and induced as described in Figure 3. After 24 h, cells were stained for the expression of TLR4 and CD14 and analysed by flow cytometry. Results show the percentage of fluorescence-positive cells (% pos cells) compared to the respective isotype controls. Statistical analysis was performed as described in Figure 3. B Analysis of CD1c, CD14 and CD19 expression on primary human CD1c+ DCs. 1×105 CD1c+ DCs were stained for the expression of CD1c, CD14 or CD19 either directly after isolation (0 h), or after 24 h culture in 24-well plates containing 1 ml of DC-medium. Cells were then analysed by flow cytometry. One representative donor is shown. Light grey, isotype control; dark grey, respective surface marker staining. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25478795), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD1c/BDCA-1

CD1c (Cluster of Differentiation antigen 1c) is a 43 kDa member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta 2‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta 2‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

Entrez Gene IDs
911 (Human)
Alternate Names
BDCA1; BDCA-1; c polypeptide; CD1; CD1A; CD1c antigen; CD1c molecule; CD1c; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; T-cell surface glycoprotein CD1c

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Citation for Human CD1c/BDCA-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Residual endotoxin contaminations in recombinant proteins are sufficient to activate human CD1c+ dendritic cells.
    Authors: Schwarz H, Schmittner M, Duschl A, Horejs-Hoeck J
    PLoS ONE, 2014-12-05;9(12):e113840.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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