Human CD31/PECAM-1 Antibody

Detection of CD31 in Human Prostate Cancer via seqIF™ staining on COMET™ CD31 Antibody was detected in immersion fixed paraffin-embedded sections of human Prostate Cancer using Rabbit Anti-Human CD31, Monoclonal Antibody (Catalog #MAB11678) at 8ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of endothelial cells. Protocol available in COMET™ Panel Builder.​​

Detection of Human CD31/PECAM‑1 by Western Blot. Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11678) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD31/PECAM‑1 in Human Colon. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11678) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD31/PECAM‑1 in Human Kidney. CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11678) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CD31/PECAM‑1 in HUVEC cells CD31/PECAM‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells (Positive) and absent in U251‑MG human malignant glioblastoma cell line (Negative) using Rabbit Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # MAB11678) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.