Human CD43 APC-conjugated Antibody

Name: Human CD43 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB2038A

Catalog #: FAB2038A Datasheet / COA / SDS

Discontinued Product

FAB2038A has been discontinued.

View all CD43 products.

Detection of CD43 in Human PBMCs by Flow Cytometry.

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All CD43 Antibodies

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Human CD43 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD43. Stains human CD43-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 290111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD43
Ser20-Pro400
Accession # P16150

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD43 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD43 APC-conjugated Monoclonal Antibody (Catalog # FAB2038A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD43

CD43, also known as leukosialin and sialophorin and Ly-48 in mouse, is a type I transmembrane sialated mucin that is expressed on most leukocytes and some tumor cells. CD43 restricts leukocyte adhesion and modulates T cell activation.

Long Name

Cluster of Differentiation 43

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Alternate Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN