Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 628211
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD96
Val22-Asp501
Accession # NP_005807

Specificity

Detects human CD96v2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD96 v2 APC‑conjugated Antibody

Detection of CD96v2 antibody in Human PBMC Lymphocytes antibody by Flow Cytometry.

Detection of CD96v2 in Human PBMC Lymphocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human CD96v2 APC-conjugated Monoclonal Antibody (Catalog # FAB6199A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD96 v2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD96

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4+ and CD8+ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155+ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Additional CD96 Products

Product Documents for Human CD96 v2 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD96 v2 APC‑conjugated Antibody

For research use only

Citations for Human CD96 v2 APC‑conjugated Antibody

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