CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 934660
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Product Specifications
Immunogen
E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002
Asn37-Phe142
Accession # Q14002
Specificity
Detects human CEACAM-7 in direct ELISAs. Stains human CEACAM-7 transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CEACAM-7 Antibody (934660)
Detection of CEACAM‑7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB4478) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human CEACAM-7 Antibody (934660)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CEACAM-7
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 7
Alternate Names
CEACAM7, CGM2
Entrez Gene IDs
1087 (Human)
Gene Symbol
CEACAM7
UniProt
Additional CEACAM-7 Products
Product Documents for Human CEACAM-7 Antibody (934660)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CEACAM-7 Antibody (934660)
For research use only
Related Research Areas
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