Human Cerebellin-4 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF6740R Datasheet / COA / SDS

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AF6740R-100UG

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Human Cerebellin-4 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cerebellin-4 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Cerebellin-1 is observed, and less than 5% cross-reactivity with rhCerebellin-2 and rhCerebellin-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-4
Gln28-Leu201
Accession # Q9NTU7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cerebellin-4

CBLN4 (Cerebellin-4) is a 26-35 kDa, secreted glycoprotein member of the C1q/TNF Superfamily of molecules. It is expressed in developing Sertoli cells and select neurons in the dorsal raphe, entorhinal cortex and arcuate nucleus. In the testis, it likely promotes cell differentiation; in the brain, it is possible that it participates in synapse formation via an interaction with neurexin. Mature human CBLN4 is 174 amino acids (aa) in length (aa 28-201). It contains two N-terminal cysteines that mediate homohexamer formation, and a C-terminal C1q domain (aa 66-201) that promotes homotrimer formation. CBLN4 also reportedly has the potential to multimerize with the other CBLN-related molecules, CBLN1-3. Mature human CBLN4 shares 99% aa identity with mouse CBLN4.

Entrez Gene IDs

140689 (Human); 228942 (Mouse); 499947 (Rat)

Alternate Names

CBLN4; CBLNL1; cerebellin 4 precursor; cerebellin precursor-like 1; Cerebellin4; Cerebellin-4; Cerebellin-like glycoprotein 1; dJ885A10.1