Human Chromogranin A Antibody (948128)

R&D Systems | Catalog # MAB90981

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 948128
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived human Chromogranin A 
Met1-Gly457
Accession # P10645

Specificity

Detects human Chromogranin A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Chromogranin A Antibody (948128)

Chromogranin A antibody in H69AR Human Cell Line by Immunocytochemistry (ICC).

Chromogranin A in H69AR Human Cell Line.

Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromogranin A antibody in Human Parathyroid by Immunohistochemistry (IHC-P).

Chromogranin A in Human Parathyroid.

Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunofluorescent Staining of Adult Stem Cell-derived Human Descending Colon Organoids.

Adult stem cells isolated from human descending colon were cultured following the steps detailed in the human intestinal organoid culture protocol. (A) The organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) The organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification.

Applications for Human Chromogranin A Antibody (948128)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed H69AR human small cell lung cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human parathyroid

Reviewed Applications

Read 1 review rated 5 using MAB90981 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Entrez Gene IDs

1113 (Human); 12652 (Mouse); 24258 (Rat)

Gene Symbol

CHGA

UniProt

P10645

Additional Chromogranin A Products

Product Documents for Human Chromogranin A Antibody (948128)

Certificate of Analysis

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Product Specific Notices for Human Chromogranin A Antibody (948128)

For research use only

Citations for Human Chromogranin A Antibody (948128)

Customer Reviews for Human Chromogranin A Antibody (948128) (1)

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  • Human Chromogranin A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tumor tissue and Prostatic adenocarcinoma tissue
    Species: Human
    Verified Customer | Posted 12/07/2021
    Human Chromogranin A Antibody (948128) MAB90981

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