Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 347625
View all CNP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CNP
Lys24-Cys126
Accession # P23582

Specificity

Detects human CNP Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-react with recombinant human (rh) ANP precursor or rhBNP precursor is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human CNP Propeptide Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Recombinant Human CNP Propeptide

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CNP

CNP belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49 aa propeptide (aa 24‑72) and a 53 aa mature peptide (aa 74‑126). Additional cleavage of the mature peptide generates 29 aa (aa 98‑126) and 22 aa (aa 105‑126) peptides, which have comparable activity in some assays. The human CNP prohormone shares 91% and 94% aa sequence identity with mouse and rat, respectively.

Long Name

C-type Natriuretic Peptide

Alternate Names

NPPC

Entrez Gene IDs

4880 (Human)

Gene Symbol

NPPC

UniProt

P23582

Additional CNP Products

Product Documents for Human CNP Propeptide Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CNP Propeptide Antibody

For research use only

Customer Reviews for Human CNP Propeptide Antibody

There are currently no reviews for this product. Be the first to review Human CNP Propeptide Antibody and earn rewards!

Have you used Human CNP Propeptide Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies