Collagen XXIII alpha 1 (sometimes abbreviated COL23A1) is a ~75 kDa type II transmembrane nonfibrillar collagen that is a member of the collagenous transmembrane protein superfamily (1, 2). This family also includes collagens XIII, XVII, XXV and non‑collagens with triple‑helical regions such as ectodysplasin A, class A macrophage scavenger receptors, and MARCO (2, 3). The human Collagen XXIII mRNA encodes a 540 amino acid (aa) protein containing a 34 aa N‑terminal cytoplasmic domain, a 21 aa transmembrane (TM) domain and a 485 aa extracellular domain (ECD). The ECD contains a coiled‑coil consensus sequence to aid homotrimerization (aa 64‑69), a furin cleavage site (aa 105‑110), a pair of cysteines thought to form intermolecular disulfides (aa 106 and 108), and three collagen domains (1, 3‑5). The C‑terminal 20 aa, including cysteines at aa 525 and 537 of Collagen XXIII, is conserved among TM collagen proteins. Proteolytic cleavage, occurrs mainly in the Golgi, and allows the Collagen XXIII ectodomain to be secreted as a soluble trimer of ~60 kDa subunits (1, 6). Cell surface cleavage can also occur, but is slow, presumably due to the presence of Collagen XXIII in lipid raft membrane domains (6). The protein database includes three variants of 537, 316 and 309 aa with various portions missing or substituted; all appear to lack TM segments (7). The human Collagen XXIII ECD shares 92%, 93%, and 91% aa sequence identity with mouse, rat, and canine Collagen XXIII, respectively. Collagen XXIII is concentrated at sites of cell contact in epithelia, and is now known to bind to Integrin alpha 2 beta 1 on the surface of stratum basale keratinocytes (2, 5, 8).
Human Collagen XXIII alpha 1 Alexa Fluor® 647‑conjugated Antibody
R&D Systems | Catalog # IC4165R
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu111-Lys540
Accession # Q86Y22
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Collagen XXIII alpha 1 Alexa Fluor® 647‑conjugated Antibody
Detection of Collagen XXIII alpha 1 in NS0 Mouse Cell Line Transfected with Human Collagen XXIII alpha 1 by Flow Cytometry.
NS0 mouse myeloma cell line transfected with human Collagen XXIII a1 was stained with Sheep Anti-Human Collagen XXIII a1 Alexa Fluor® 647-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4165R, filled histogram) or isotype control antibody (Catalog # IC016R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human Collagen XXIII alpha 1 Alexa Fluor® 647‑conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: NS0 mouse myeloma cell line transfected with human Collagen XXIII alpha 1 were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
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Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Collagen XXIII alpha 1
References
- Banyard, J. et al. (2003) J. Biol. Chem. 278:20989.
- Franzke, C-W. et al. (2005) J. Biol. Chem. 280:4005.
- Ricard-Blum, S. et al. (2011) Cold Spring Harb. Prespect. Biol. 3:a004978.
- Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
- Koch, M. et al. (2006) J. Biol. Chem. 281:21546.
- Veit, G. et al. (2007) J. Biol. Chem. 282:27424.
- Entrez Accession # EAW53833, EAW53834, and AAH42428.
- Veit, G. et al. (2011) J. Biol. Chem. 286:27804.
Alternate Names
Gene Symbol
UniProt
Additional Collagen XXIII alpha 1 Products
Product Documents for Human Collagen XXIII alpha 1 Alexa Fluor® 647‑conjugated Antibody
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Product Specific Notices for Human Collagen XXIII alpha 1 Alexa Fluor® 647‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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