Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1080201
View all Cytokeratin 17/19 Primary Antibodies »

Product Specifications

Immunogen

Cytokeratin 17/19 containing synthetic peptide
Accession # Q04695

Specificity

Detects a synthetic peptide around aa 123 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human Cytokeratin 17/19 Antibody

Detection of Human Cytokeratin 17/19 by Western Blot.

Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HCC827 human non-small cell lung cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Cytokeratin 17 at approximately 48 kDa and for Cytokeratin 19 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Cytokeratin 17/19 in Human Prostate.

Cytokeratin 17/19 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of basal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Cytokeratin 17/19 in Human Squamous Cell Carcinoma.

Cytokeratin 17/19 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Cytokeratin 17/19 by Simple WesternTM.

Simple Western shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.5 mg/ml. Specific bands were detected for Cytokeratin 17 at approximately 53kDa and for Cytokeratin 19 at approximately 51kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Cytokeratin 17/19 Monoclonal Antibody (Catalog # MAB11568). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Cytokeratin 17/19 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate and human squamous cell carcinoma

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MDA-MB-468 human breast cancer cell line

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HCC827 human non-small cell lung cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 17/19

Cytokeratin 17 (KRT17) is a 48 kDa, 432 aa type I keratin that has been studied in several types of cancer. KRT17 promotes epithelial cell proliferation and tumor growth in skin. Many studies have shown KRT17 overexpression in many cancers including cervical, oral, ovarian, gastric, lung and pancreatic cancer among others. In certain types of  breast cancer, KRT17 overexpression has been associated with poor prognosis. KRT17 expression is closely associated with prognosis in cancer and can be a novel therapeutic target.

References

  1. Tang S, Liu W, Yong L, Liu D, Lin X, Huang Y, Wang H, Cai F. Reduced Expression of KRT17 Predicts Poor Prognosis in HER2high Breast Cancer. Biomolecules. 2022 Aug 25;12(9):1183. doi: 10.3390/biom12091183. PMID: 36139022; PMCID: PMC9496156.
  2. Hu H, Xu DH, Huang XX, Zhu CC, Xu J, Zhang ZZ, Zhao G. Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer. J Cancer. 2018 Jan 1;9(2):346-357. doi: 10.7150/jca.19838. PMID: 29344281; PMCID: PMC5771342. 
  3. Li D, Ni XF, Tang H, Zhang J, Zheng C, Lin J, Wang C, Sun L, Chen B. KRT17 Functions as a Tumor Promoter and Regulates Proliferation, Migration and Invasion in Pancreatic Cancer via mTOR/S6k1 Pathway. Cancer Manag Res. 2020 Mar 19;12:2087-2095. doi: 10.2147/CMAR.S243129. PMID: 32256116; PMCID: PMC7090205.

Alternate Names

CK-19, K1CS, keratin 19, type I, KRT17

Entrez Gene IDs

3872 (Human); 16667 (Mouse); 287702 (Rat)

Gene Symbol

Q04695

UniProt

Q04695 (Human)

Additional Cytokeratin 17/19 Products

Product Documents for Human Cytokeratin 17/19 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cytokeratin 17/19 Antibody

For research use only

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Protocols

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