Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LP3K
View all Cytokeratin 8 Primary Antibodies »

Product Specifications

Immunogen

SV-40 transformed human neonatal keratinocyte extract

Specificity

Detects human Cytokeratin 8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cytokeratin 8 Antibody

Cytokeratin 8 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Cytokeratin 8 in MCF‑7 Human Cell Line.

Cytokeratin 8 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cytokeratin 8 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

Cytokeratin 8 in Human Colon Cancer Tissue.

Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cytokeratin 8 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LS180 human colorectal adenocarcinoma cell line and immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Western Blot

Lane, E.B. et al. (1985) Ann. NY Acad. Sci. 455:241. This application was not tested by R&D Systems.

Reviewed Applications

Read 7 reviews rated 4.1 using MAB3165 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 8

Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

Alternate Names

CARD2, CK8, EndoA, KRT8

Entrez Gene IDs

3856 (Human); 16691 (Mouse); 25626 (Rat)

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Human Cytokeratin 8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cytokeratin 8 Antibody

For research use only

Citations for Human Cytokeratin 8 Antibody

Customer Reviews for Human Cytokeratin 8 Antibody (7)

4.1 out of 5
7 Customer Ratings
5 Stars
43%
4 Stars
29%
3 Stars
29%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 7 reviews Showing All
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  • Human Cytokeratin 8 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human proximal tubular epithelial cells
    Species: Human
    Verified Customer | Posted 11/08/2021
    Human Cytokeratin 8 Antibody MAB3165
  • Human Cytokeratin 8 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Cytokeratin 8 Antibody MAB3165
  • Human Cytokeratin 8 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Cytokeratin 8 Antibody MAB3165
  • Human Cytokeratin 8 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human Cytokeratin 8 Antibody
    Name: simon liu
    Application: Immunohistochemistry
    Sample Tested: Kidney cancer tissue
    Species: Human
    Verified Customer | Posted 12/10/2017
    Human Cytokeratin 8 Antibody MAB3165
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24572574
    Species: Mouse
    Verified Customer | Posted 02/19/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24572574
    Species: Human
    Verified Customer | Posted 02/19/2015

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Protocols

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