Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (638708)

Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cytosolic Sulfotransferase 1A1/SULT1A1 in Human Brain.

Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot

SULT1A1 is highly expressed in primary human monocyte-derived macrophages (MDMs). a The cellular sulfonation pathway. The first step of the cellular sulfonation pathway involves import through a sulfate transporter of a sulfate ion that is then used as a substrate by either 3′-phosphoadenosine-5′phosphosulfate (PAPS) synthetase enzymes PAPSS1 or PAPSS2. These proteins catalyze two enzymatic steps to generate PAPS, the high-energy universal sulfonate-donor from sulfate and two molecules of ATP. PAPS can be transported across the Golgi membrane and used by the Golgi sulfotransferases to generate sulfonated proteins, glycoproteins, glycoproteins, glycolipids, and proteoglycans. Alternatively, PAPS can be used by cytosolic sulfotransferases (SULTS) to sulfonate small molecules such as hormones, neurotransmitters, and xenobiotics. b Human CD4+ T cells and CD14+ monocytes were isolated from donor PBMCs by magnetic bead isolation. Resting CD4+ T cells were lysed directly after separation, and the remaining CD4+ T cells were activated using CD3/CD28 beads for three days. Monocytes were cultured for 7 days in the presence of 20 ng/ml M-CSF, were lysed, subjected to gel electrophoresis, and immunoblotting was performed to detect SULT1A1 or the loading control Ku86 protein Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26906565), licensed under a CC-BY license. Not internally tested by R&D Systems.