Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (844529)

R&D Systems | Catalog # MAB7458

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 844529
View all Cytosolic Sulfotransferase 1C2/SULT1C2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1C2/SULT1C2
Ala2-Leu296
Accession # O00338

Specificity

Detects human Cytosolic Sulfotransferase 1C2/SULT1C2 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (844529)

Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1C2/SULT1C2 Monoclonal Antibody (Catalog # MAB7458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 1C2/SULT1C2 antibody in Human Stomach by Immunohistochemistry (IHC-P).

Cytosolic Sulfotransferase 1C2/SULT1C2 in Human Stomach.

Cytosolic Sulfotransferase 1C2/SULT1C2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Cytosolic Sulfotransferase 1C2/SULT1C2 Monoclonal Antibody (Catalog # MAB7458) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (844529)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1C2/SULT1C2

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869.
  4. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.

Alternate Names

ST1C1, ST1C2, SULT1C1, SULT1C2

Entrez Gene IDs

6819 (Human); 316153 (Rat)

Gene Symbol

SULT1C2

UniProt

O00338 (Human)

Additional Cytosolic Sulfotransferase 1C2/SULT1C2 Products

Product Documents for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (844529)

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 1C2/SULT1C2 Antibody (844529)

For research use only

Related Research Areas

Sulfation Regulators

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Protocols

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