Human Cytosolic Sulfotransferase 4A1/SULT4A1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5826T Datasheet / COA / SDS

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AF5826T-100UG

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All Cytosolic Sulfotransferase 4A1/SULT4A1 Antibodies

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Human Cytosolic Sulfotransferase 4A1/SULT4A1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SULT4A1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT2A1, and rhSULT1E1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SULT4A1 isoform 1
Ala2-Leu284
Accession # Q9BR01

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cytosolic Sulfotransferase 4A1/SULT4A1

SULT4A1 (Cytosolic Sulfotransferase 4A1; also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A1 is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284 amino acids (aa) in length. A weak PAPS binding site is found between aa 246-254. There are two potential isoforms; one that shows a 13 aa substitution for aa 248-284 and a second that contains a 48 aa substitution for aa 202 - 247. Full-length human SULT4A1 is 98% aa identical to mouse SULT4A1.

Entrez Gene IDs

25830 (Human); 29859 (Mouse)

Alternate Names

Brain sulfotransferase-like protein; BRSTL1; BR-STL-1; Cytosolic Sulfotransferase 4A1; EC 2.8.2.-; hBR-STL; hBR-STL-1MGC40032; nervous system cytosolic sulfotransferase; Nervous system sulfotransferase; NST; ST4A1; sulfotransferase 4A1; sulfotransferase family 4A, member 1; sulfotransferase-related protein; SULT4A1; SULTX3; SULTX3DJ388M5.3