Human Decorin Antibody (115413) [Biotin]

R&D Systems | Catalog # BAM1431

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Array Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 115413
View all Decorin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys172
Accession # NP_598013.1

Specificity

Detects human Decorin in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Decorin Antibody (115413) [Biotin]

Human Decorin ELISA Standard Curve

Human Decorin ELISA Standard Curve

Recombinant Human Decorin (Catalog # 143-DE) was serially diluted and captured by Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB1432) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Decorin Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Decorin Antibody (115413) [Biotin]

Human Decorin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Decorin Antibody (Catalog # MAB1432)
  • Standard: Recombinant Human Decorin Protein, CF (Catalog # 143-DE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 - 12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

References

  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Entrez Gene IDs

1634 (Human); 13179 (Mouse)

Gene Symbol

DCN

UniProt

NP_598013.1

Additional Decorin Products

Product Documents for Human Decorin Antibody (115413) [Biotin]

Certificate of Analysis

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Product Specific Notices for Human Decorin Antibody (115413) [Biotin]

For research use only

Citations for Human Decorin Antibody (115413) [Biotin]

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