Human Desmin Antibody

Detection of Desmin in Human Skeletal Muscle via seqIF™ staining on COMET™ Desmin was detected in immersion fixed paraffin-embedded sections of human Skeletal Muscle using Rabbit Anti-Human Monoclonal Antibody (Catalog # MAB11654) at 0.1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse and Rat Desmin by Western Blot. Western Blot shows lysates of RD human rhabdomyosarcoma cell line, C2C12 mouse myoblast cell line and rat heart tissue. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11654) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Desmin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11654) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Desmin in Human Skeletal Muscle. Desmin was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Rabbit Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11654) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.