Human Desmocollin‑1 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB4955AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2906A
View all Desmocollin-1 Primary Antibodies »

Product Specifications

Specificity

Detects human Desmocollin-1 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human Desmocollin‑1 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Desmocollin-1

Desmocollin-1 (DSC1) is a 100 kDa-110 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules (1-3). Human DSC1 is synthesized with a 105 amino acid (aa) propeptide. The mature protein consists of a 557 aa extracellular domain (ECD) that contains five cadherin-like domains, a 23 aa transmembrane segment, and a 180 aa cytoplasmic domain (4, 5). Within the ECD, human DSC1 shares 79% and 82% aa sequence identity with bovine and mouse DSC1, respectively, and 53% with human DSC2 and DSC3. An alternately spliced isoform has a substituted and truncated cytoplasmic domain (5). DSC1 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is predominantly expressed in the outer layers of stratified epithelia of the skin, tongue, and hair follicle root sheath (6, 7). It is required for both promoting epidermal differentiation and maintaining epidermal strength (8). DSC2 and DSC3, by contrast, are preferentially localized in the basal and suprabasal layers of the epidermis (1). DSC1 is not expressed in normal colon epithelium, but it is induced during colon carcinogenesis (9). Loss of DSC1 function in the skin disorders IgA pemphigus and Netherton Syndrome results from autoimmune targeting or enhanced KLK-7 mediated proteolysis, respectively (10-12).

References

  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. King, I.A. et al. (1993) Genomics 18:185.
  5. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
  6. King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.
  7. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
  8. Chidgey, M. et al. (2001) J. Cell Biol. 155:821.
  9. Khan, K. et al. (2006) Br. J. Cancer 95:1367.
  10. Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.
  11. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  12. Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.

Alternate Names

CDHF1, Desmocollin1, DG2/DG3, DSC1

Entrez Gene IDs

1823 (Human); 13505 (Mouse); 291759 (Rat)

Gene Symbol

DSC1

UniProt

Q08554 (Human)

Additional Desmocollin-1 Products

Product Documents for Human Desmocollin‑1 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Desmocollin‑1 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Cadherin Superfamily

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Protocols

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