Human DOT1L Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB6546U Datasheet / COA / SDS

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FAB6546U-100UG

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Human DOT1L Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.

Source

Monoclonal Mouse IgG2a Clone # 653613

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DOT1L
Gly2-Lys416
Accession # Q8TEK3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DOT1L

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Long Name

Disruptor of Telomeric Silencing 1-like

Entrez Gene IDs

84444 (Human)

Alternate Names

DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4