Human EGLN3/PHD3 Antibody (700210)

R&D Systems | Catalog # MAB6954

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 700210
View all EGLN3/PHD3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EGLN3/PHD3
Pro2-Asp239
Accession # Q9H6Z9

Specificity

Detects human EGLN3/PHD3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) EGLN1/PHD2 or rhEGLN2/PHD1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EGLN3/PHD3 Antibody (700210)

EGLN3/PHD3 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

EGLN3/PHD3 in A549 Human Cell Line.

EGLN3/PHD3 was detected in immersion fixed A549 human lung carcinoma cell line, untreated (lower panel) or treated (upper panel) with CoCl2, using Mouse Anti-Human EGLN3/PHD3 Monoclonal Antibody (Catalog # MAB6954) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human EGLN3/PHD3 Antibody (700210)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB6954 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGLN3/PHD3

Egl nine homolog 3 (EGLN3), also known as PHD3, is a widely expressed 27 kDa enzyme that hydroxylates proline residues on target proteins including HIF-1 alpha. HIF-1 is an alpha / beta heterodimeric transcriptional activator that upregulates genes involved in mitigating the effects of hypoxia. Normally, and in the presence of abundant oxygen, the HIF-1 alpha -chain is hydroxylated by PHD family members, which results in its ubiquitylation and degradation. Under low oxygen tension, EGLN3 activity is decreased, the HIF-1 alpha subunit is retained, and HIF-1 activates genes. EGLN3 also hydroxylates and promotes the degradation of the beta -2-adrenergic receptor, promotes myogenic differentiation, promotes apoptosis via caspase activation, and blocks tumor angiogenesis. EGLN3 forms homomultimers and heteromultimers with other EGLN proteins, and this is enhanced during hypoxia. EGLN3 contains one iron 2-oxoglutarate (Fe2OG) dioxygenase domain (aa 278-376), an iron-binding site (Asp 137 and His196), and a 2-oxoglutarate-binding site (Arg205). Within aa 2-239, human EGLN3 shares 97% aa sequence identity with mouse and rat EGLN3.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Entrez Gene IDs

112399 (Human); 112407 (Mouse); 54702 (Rat)

Gene Symbol

EGLN3

UniProt

Q9H6Z9

Additional EGLN3/PHD3 Products

Product Documents for Human EGLN3/PHD3 Antibody (700210)

Certificate of Analysis

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Product Specific Notices for Human EGLN3/PHD3 Antibody (700210)

For research use only

Customer Reviews for Human EGLN3/PHD3 Antibody (700210) (1)

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  • Human EGLN3/PHD3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/16/2022
    Human EGLN3/PHD3 Antibody (700210) MAB6954

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Associated Pathways

HIF Enhancer Pathways
HIF Enhancer Pathway Thumbnail