Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Endorepellin/Perlecan Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Endorepellin/Perlecan
Arg3684-Ser4391 (Ser4003Thr)
Accession # P98160

Specificity

Detects human Endorepellin/Perlecan in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Endorepellin/Perlecan Antibody

Endorepellin/Perlecan antibody in Human Skin by Immunohistochemistry (IHC-P).

Endorepellin/Perlecan in Human Skin.

Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/ Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Endorepellin/Perlecan Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Endorepellin/Perlecan

Reviewed Applications

Read 5 reviews rated 4 using AF2364 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endorepellin/Perlecan

Endorepellin is the C-terminal domain of the heparan sulfate proteoglycan perlecan, which is a modular protein found in basement membranes. Endorepellin has been found to be an inhibitor of angiogenesis that blocks endothelial cell adhesion to fibronectin and type I collagen.

Long Name

Heparan Sulfate Proteoglycan

Alternate Names

HSPG2, Perlecan

Entrez Gene IDs

3339 (Human); 15530 (Mouse)

Gene Symbol

HSPG2

UniProt

P98160

Additional Endorepellin/Perlecan Products

Product Documents for Human Endorepellin/Perlecan Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Endorepellin/Perlecan Antibody

For research use only

Citations for Human Endorepellin/Perlecan Antibody

Customer Reviews for Human Endorepellin/Perlecan Antibody (5)

4 out of 5
5 Customer Ratings
5 Stars
20%
4 Stars
60%
3 Stars
20%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 5 reviews Showing All
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  • Human Endorepellin/Perlecan Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: bEnd.3 mouse endothelioma cell line
    Species: Mouse
    Verified Customer | Posted 11/27/2020
  • Human Endorepellin/Perlecan Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: RAT SERUM
    Verified Customer | Posted 08/23/2020
    Human Endorepellin/Perlecan Antibody AF2364
  • Human Endorepellin/Perlecan Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human Endorepellin/Perlecan Antibody AF2364
  • Human Endorepellin/Perlecan Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human Endorepellin/Perlecan Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

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Protocols

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