Human Enolase 2/Neuron-specific Enolase Antibody (972458)

R&D Systems | Catalog # MAB51691

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 972458
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Enolase 2/Neuron‑specific Enolase
Met1-Leu434
Accession # P09104

Specificity

Detects human Enolase 2/Neuron‑specific Enolase in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Enolase 2/Neuron-specific Enolase Antibody (972458)

Detection of Human, Mouse, and Rat Enolase 2/Neuron-specific Enolase antibody by Western Blot.

Detection of Human, Mouse, and Rat Enolase 2/Neuron‑specific Enolase by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB51691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Enolase 2/Neuron-specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Enolase 2/Neuron-specific Enolase antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

Enolase 2/Neuron‑specific Enolase in SH‑SY5Y Human Cell Line.

Enolase 2/Neuron-specific Enolase was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB51691) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Enolase 2/Neuron-specific Enolase Antibody (972458)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Enolase 2/Neuron-specific Enolase

Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Entrez Gene IDs

2026 (Human); 13807 (Mouse); 24334 (Rat)

Gene Symbol

ENO2

UniProt

P09104

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Human Enolase 2/Neuron-specific Enolase Antibody (972458)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Enolase 2/Neuron-specific Enolase Antibody (972458)

For research use only

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Customer Reviews for Human Enolase 2/Neuron-specific Enolase Antibody (972458)

There are currently no reviews for this product. Be the first to review Human Enolase 2/Neuron-specific Enolase Antibody (972458) and earn rewards!

Have you used Human Enolase 2/Neuron-specific Enolase Antibody (972458)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies