Human ERp57/PDIA3 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF8219S Datasheet / COA / SDS

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AF8219S-100UG

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All ERp57/PDIA3 Antibodies

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Human ERp57/PDIA3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERp57/PDIA3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant ERp57/PDIA3
Arg107-Lys366
Accession # P30101

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ERp57/PDIA3

ERp57 (Endoplasmic reticulum resident protein 57; also Protein disulfide-isomerase A3/PDIA3) is a 57-60 kDa member of the protein disulfide isomerase family of proteins. Knockdown of ERp57 or antibody-targeted inhibition of the secreted form significantly impaired the secretion and accumulation of extracellular matrix. ERp57 is highly similar to other PDI family members in amino acid sequence and structural/functional domain organization. This protein plays an important role in endoplasmic reticulum quality control of newly synthesized glycoproteins, is critical in major histocompatability complex class I assembly and regulates gene expression. ERp57 has been implicated in human pathologies including cancer and Alzheimer's disease. Within aa 107 - 366, human ERp57 shares 92% aa sequence identity with mouse ERp57.

Long Name

Endoplasmic Reticulum Resident Protein 57

Entrez Gene IDs

2923 (Human); 14827 (Mouse); 29468 (Rat)

Alternate Names

Disulfide isomerase ER-60; EC 5.3.4.1; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER protein 60; ER60; ERp57; ERp5758 kDa glucose-regulated protein; ERp60; ERp6058 kDa microsomal protein; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; GRP58ER protein 57; HsT17083; P58; PDIA3; phospholipase C-alpha; PI-PLC; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3