Human FGF-21 Antibody

R&D Systems | Catalog # AF2539

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FGF-21
His29-Ser209
Accession # Q9NSA1

Specificity

Detects human FGF-21 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse FGF-21 is observed and less than 2% cross‑reactivity with recombinant human FGF-19 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FGF-21 Antibody

Human FGF‑21 ELISA Standard Curve.

Recombinant Human FGF‑21 protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994.
Human FGF-21 ELISA Standard Curve

Human FGF-21 ELISA Standard Curve

Recombinant Human FGF‑21 (Catalog # 2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human FGF-21 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FGF-21 DuoSet ELISA Kit (Catalog # DY2539) for convenient development of a sandwich ELISA or the Human FGF-21 Quantikine ELISA Kit (Catalog # DF2100) for  a complete optimized ELISA.

Western Blot

0.1 µg/mL
Sample: Recombinant Human FGF‑21 (Catalog # 2539-FG)

Reviewed Applications

Read 1 review rated 5 using AF2539 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the
C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6).
The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a
high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

References

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Huang, X. et al. (2006) Mol. Carcinog. 45:934.
  4. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  5. GenBank Accession #: EAW52401 (2006).
  6. Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
  7. Moore, D. D. (2007) Science 316:1436.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  9. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
  10. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
  11. Badman, M.K. et al. (2007) Cell Metab. 5:426.
  12. Inagaki, T. et al. (2007) Cell Metab. 5:415.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Entrez Gene IDs

26291 (Human); 56636 (Mouse)

Gene Symbol

FGF21

UniProt

Q9NSA1

Additional FGF-21 Products

Product Documents for Human FGF-21 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGF-21 Antibody

For research use only

Citations for Human FGF-21 Antibody

Customer Reviews for Human FGF-21 Antibody (1)

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  • Human FGF-21 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Heparin Plasma
    Species: Human
    Verified Customer | Posted 05/13/2021
    This antibody served as a capture in an immunoassay measuring FGF21 in human plasma samples.
    Human FGF-21 Antibody AF2539

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