Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 98725
View all FGFR2 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms

Specificity

Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta  isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGFR2 APC‑conjugated Antibody

Detection of FGF R2 antibody in KATO-III Human Cell Line antibody by Flow Cytometry.

Detection of FGF R2 in KATO-III Human Cell Line by Flow Cytometry.

KATO-III human gastric carcinoma cell line was stained with Mouse Anti-Human FGF R2 APC-conjugated Monoclonal Antibody (Catalog # FAB684A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human FGFR2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: KATO‑III human gastric carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FGFR2

FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha  isoforms) or two ( beta  isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Human FGFR2 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FGFR2 APC‑conjugated Antibody

For research use only

Citations for Human FGFR2 APC‑conjugated Antibody

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