Human FGL2 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB5974U Datasheet / COA / SDS

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FAB5974U-100UG

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Human FGL2 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGL2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-like 2, and recombinant mouse Angiopoietin-3 is observed.

Source

Monoclonal Mouse IgG2b Clone # 532223

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FGL2
Asn24-Pro439
Accession # Q14314

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGL2

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64‑70 kDa secreted glycoprotein of the fibrinogen-like superfamily. It is produced by macrophages and endothelial cells in response to inflammatory mediators, T cells and fetal trophoblast cells. FGL2 has prothrombinase activity, promotes immune-mediated thrombosis and functions in transplant rejection and infection-related abortion. Mature human FGL2 gene is a 416 amino acid (aa) protein with a coiled-coil region and a fibronectin C-terminal homology domain or FRED. Coiled-coil dimers are covalently linked to form a tetrameric 260‑280 aa FGL2 complex. Mature human FGL2 shares 79% aa identity with mouse FGL2.

Long Name

Fibrinogen-like 2

Entrez Gene IDs

10875 (Human); 14190 (Mouse); 84586 (Rat); 102123631 (Cynomolgus Monkey)

Alternate Names

FGL2; fibrinogen-like 2; Fibrinogen-like protein 2; Fibroleukin; pT49T49; T49