Human Fibromodulin/FMOD PE‑conjugated Antibody

R&D Systems | Catalog # IC5945P

R&D Systems
Discontinued Product
IC5945P has been discontinued. View all Fibromodulin/FMOD products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 549302
View all Fibromodulin/FMOD Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
Asp75-Ile376
Accession # NP_002014

Specificity

Detects human Fibromodulin/FMOD in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Lumican is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Fibromodulin/FMOD PE‑conjugated Antibody

Detection of Fibromodulin/FMOD antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

Detection of Fibromodulin/FMOD in MG-63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human Fibromodulin/FMOD PE-conjugated Monoclonal Antibody (Catalog # IC5945P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Fibromodulin/FMOD PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MG‑63 human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50‑70 kDa protein contains a sulfated N‑terminal complement C1q binding domain (aa 19‑98) and 12 leucine-rich repeats (aa 75‑376). A 40 kDa form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa sequence identity with mouse and rat FMOD.

Alternate Names

FM, FMOD, SLRR2E

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Gene Symbol

FMOD

UniProt

NP_002014

Additional Fibromodulin/FMOD Products

Product Documents for Human Fibromodulin/FMOD PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Fibromodulin/FMOD PE‑conjugated Antibody

For research use only

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail