Human Flt-3/Flk-2 Antibody
Human Flt-3/Flk-2 Antibody Summary
Accession # AAA18947
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Flt‑3/Flk‑2 in THP-1 cells by Flow Cytometry. THP-1 cells were stained with Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB812, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of Flt‑3/Flk‑2 in PBMC monocytes by Flow Cytometry. PBMC monocytes were stained with Mouse Anti-Human Flt‑3/Flk‑2 Monoclonal Antibody (Catalog # MAB812, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.
Citations for Human Flt-3/Flk-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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FLT3-ITD transduces autonomous growth signals during its biosynthetic trafficking in acute myelogenous leukemia cells
Authors: K Yamawaki, I Shiina, T Murata, S Tateyama, Y Maekawa, M Niwa, M Shimonaka, K Okamoto, T Suzuki, T Nishida, R Abe, Y Obata
Scientific Reports, 2021-11-22;11(1):22678.
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot
Fluvastatin inhibits FLT3 glycosylation in human and murine cells and prolongs survival of mice with FLT3/ITD leukemia.
Authors: Williams A, Li L, Nguyen B, Brown P, Levis M, Small D
Sample Types: Whole Cells
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