Human FosB/G0S3 Antibody (269928)

Name: Human FosB/G0S3 Antibody (269928)
Brand: R&D Systems
SKU: MAB2214
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB2214

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 269928

View all FosB/G0S3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FosB
Met1-Leu338
Accession # P53539

Specificity

Detects human FosB in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human FosB/G0S3 Antibody (269928)

Detection of Human FosB/G0S3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with TPA for 2 hours. PVDF membrane was probed with 0.5 µg/mL of Human FosB/G0S3 Monoclonal Antibody (Catalog # MAB2214), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FosB/G0S3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FosB/G0S3 Antibody (269928)

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: TPA-treated HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FosB/G0S3

FosB (FBJ murine osteoscarcoma viral oncogene homolog B) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. AP-1 activity is important for gene transcription in a wide variety of cellular processes.