Human Fucosyltransferase 8/FUT8 Antibody
R&D Systems | Catalog # AF5768
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Asp32-Lys575
Accession # Q9BYC5
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Fucosyltransferase 8/FUT8 Antibody
Fucosyltransferase 8/FUT8 in Human Colon.
Fucosyltransferase 8/FUT8 was detected in paraffin-embedded sections of human colon using Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Detection of Human Fucosyltransferase 8/FUT8 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 60 - 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Detection of Human Fucosyltransferase 8/FUT8 by Simple WesternTM.
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fucosyltransferase 8/FUT8 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Fucosyltransferase 8/FUT8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5768) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Detection of FUT8 by Immunohistochemistry
Correlations among FUT8 and B7H3 expression in TNBC tissues.a Expression of FUT8 protein in 14 representative human TNBC fresh samples by immunoblot. N, matched normal tissue; T, tumor tissue. b The representative intensity images for each IHC score of FUT8 staining in TNBC tumor tissues were shown (left). Kaplan–Meier plots of the overall survival of patients, stratified by protein expression of FUT8 (right). The p value was assessed using the log-rank test (two-sided). c The representative images for B7H3 staining in two patients with FUT8 expression (left). Case 1 showed low expression of FUT8 with low expression of B7H3. Case 2 showed high expression of FUT8 with high expression of B7H3. The correlation of B7H3 with FUT8 expression status in TNBC patient tumors (right). The relationship was assessed using Pearson’s chi-square test. d Kaplan–Meier plots of the overall survival of patients, stratified by protein expression of both B7H3 and FUT8. The p value was assessed using the log-rank test and further corrected with the Benjamini–Hochberg method (two-sided). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33976130), licensed under a CC-BY license. Not internally tested by R&D Systems.Detection of FUT8 by Immunohistochemistry
Correlations among FUT8 and B7H3 expression in TNBC tissues.a Expression of FUT8 protein in 14 representative human TNBC fresh samples by immunoblot. N, matched normal tissue; T, tumor tissue. b The representative intensity images for each IHC score of FUT8 staining in TNBC tumor tissues were shown (left). Kaplan–Meier plots of the overall survival of patients, stratified by protein expression of FUT8 (right). The p value was assessed using the log-rank test (two-sided). c The representative images for B7H3 staining in two patients with FUT8 expression (left). Case 1 showed low expression of FUT8 with low expression of B7H3. Case 2 showed high expression of FUT8 with high expression of B7H3. The correlation of B7H3 with FUT8 expression status in TNBC patient tumors (right). The relationship was assessed using Pearson’s chi-square test. d Kaplan–Meier plots of the overall survival of patients, stratified by protein expression of both B7H3 and FUT8. The p value was assessed using the log-rank test and further corrected with the Benjamini–Hochberg method (two-sided). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33976130), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Fucosyltransferase 8/FUT8 Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8, see our available Western blot detection antibodies
Simple Western
Sample: COLO 205 human colorectal adenocarcinoma cell line
Western Blot
Sample: COLO 205 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Fucosyltransferase 8/FUT8
Alternate Names
Gene Symbol
UniProt
Additional Fucosyltransferase 8/FUT8 Products
Product Documents for Human Fucosyltransferase 8/FUT8 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Fucosyltransferase 8/FUT8 Antibody
For research use only
Related Research Areas
Citations for Human Fucosyltransferase 8/FUT8 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars