Human GAD2/GAD65 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF2247U Datasheet / COA / SDS

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AF2247U-100UG

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All GAD2/GAD65 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GAD2/GAD65 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GAD2/GAD65
Ala1-Ala150
Accession # Q05329

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GAD2/GAD65

Glutamic acid decarboxylases (GAD), which catalyze the formation of gamma-aminobutyric acid from L-glutamic acid, are detectable in different isoforms encoded by distinct genes. GAD2, also known as GAD65, is expressed in the brain and pancreatic islets. GAD2 has been implicated as an autoantigen in the autoimmune disease Stiff Man Syndrome and in insulin-dependent diabetes mellitus.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Entrez Gene IDs

2572 (Human)

Alternate Names

EC 4.1.1; EC 4.1.1.15; GAD2; GAD65; GAD-65; GAD65MGC161605; glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); MGC161607,65 kDa glutamic acid decarboxylase