Human gamma-Sarcoglycan/SGCG Antibody

Catalog #: MAB11731 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11731-100
MAB11731-SP

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Detection of Human gamma-Sarcoglycan/SGCG by Western Blot.

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Human gamma-Sarcoglycan/SGCG Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for Human SGCG around amino acid 190 in Direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1112101

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # Q13326

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Human skeletal muscle tissue

Simple Western

20 µg/mL

Human skeletal muscle tissue

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human heart and skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human gamma-Sarcoglycan/SGCG by Western Blot. Western Blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human gamma-Sarcoglycan/SGCG Monoclonal Antibody (Catalog # MAB11731) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gamma-Sarcoglycan/SGCG at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Detection of gamma-Sarcoglycan/SGCG in Human Heart. gamma-Sarcoglycan/SGCG was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human gamma-Sarcoglycan/SGCG Monoclonal Antibody (Catalog # MAB11731) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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Detection of gamma-Sarcoglycan/SGCG in Human Skeletal Muscle. gamma-Sarcoglycan/SGCG was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human gamma-Sarcoglycan/SGCG Monoclonal Antibody (Catalog # MAB11731) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human gamma-Sarcoglycan/SGCG by Simple Western^TM. Simple Western lane view shows lysates of human skeletal muscle tissue, loaded at 0.5 mg/ml. A specific band was detected for gamma-Sarcoglycan/SGCG at approximately 49 kDa (as indicated) using 20 µg/ml of Mouse Anti-Human gamma-Sarcoglycan/SGCG Monoclonal Antibody (Catalog # MAB11731) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Sarcoglycan/SGCG

Sarcoglycan gamma (SGCG) is a transmembrane protein and a key component of the dystrophin-associated glycoprotein complex (DGC), with a molecular weight of approximately 35 kDa. The sarcoglycan complex, which includes SGCG, is critical for maintaining the structural integrity of muscle cell membranes and for linking the actin cytoskeleton to the extracellular matrix. SGCG is predominantly expressed in skeletal and cardiac muscle, where it plays a crucial role in stabilizing the muscle membrane during contraction. Mutations in the SGCG gene are associated with limb-girdle muscular dystrophy type 2C (LGMD2C), a progressive muscular dystrophy characterized by muscle weakness and membrane instability. Loss of SGCG function leads to disruption of the DGC, resulting in increased susceptibility to muscle membrane damage and impaired muscle regeneration. Recent studies suggest that SGCG may also be involved in signaling pathways regulating muscle homeostasis and repair. Its critical role in muscle integrity, disease pathogenesis, and signaling underscores its potential as a therapeutic target for the treatment of muscular dystrophies.

References

Hack AA, Groh ME, McNally EM. Sarcoglycans in muscular dystrophy. Microsc Res Tech. 2000 Feb 1-15;48(3-4):167-80. doi: 10.1002/(SICI)1097-0029(20000201/15)48:3/43.0.CO;2-T. PMID: 10679964.
Groh S, Zong H, Goddeeris MM, Lebakken CS, Venzke D, Pessin JE, Campbell KP. Sarcoglycan complex: implications for metabolic defects in muscular dystrophies. J Biol Chem. 2009 Jul 17;284(29):19178-82. doi: 10.1074/jbc.C109.010728. Epub 2009 Jun 3. PMID: 19494113; PMCID: PMC2740540.
Bushby KM. The limb-girdle muscular dystrophies-multiple genes, multiple mechanisms. Hum Mol Genet. 1999;8(10):1875-82. doi: 10.1093/hmg/8.10.1875. PMID: 10469840.

Long Name

Sarcoglycan, Gamma (35kDa Dystrophin-Associated Glycoprotein)

Entrez Gene IDs

6445 (Human); 24053 (Mouse)

Alternate Names

35 kDa dystrophin-associated glycoprotein; 35DAG; A4; DAGA4; DAGA4,35kD dystrophin-associated glycoprotein; DMDA; DMDA1; gamma sarcoglycan; gamma-Sarcoglycan; gamma-SG; LGMD2C; LGMDR5; MAM; MGC130048; sarcoglycan, gamma (35kD dystrophin-associated glycoprotein); sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein); SCARMD2; SCG3; SCG3LGMD2C; TYPE