Human GITR/TNFRSF18 Antibody (110416)

R&D Systems | Catalog # MAB689

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 110416
View all GITR/TNFRSF18 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5

Specificity

Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse(rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human GITR/TNFRSF18 Antibody (110416)

Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry

RPMI8226 cells (filled histogram) vs. Jurkat cells (open histogram) were stained with Mouse Anti-Human GITR/TNFRSF18 Monoclonal Antibody (Catalog # MAB689) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Human GITR / TNFRSF18 ELISA Standard Curve

Human GITR / TNFRSF18 ELISA Standard Curve

Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) was serially diluted and captured by Mouse Anti-Human GITR/TNFRSF18 Monoclonal Antibody (Catalog # MAB689) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GITR/TNFRSF18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF689) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human GITR/TNFRSF18 Antibody (110416)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-2 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human GITR Ligand/TNFSF18 (Catalog # 694-GL) to immobilized Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR) coated at 2 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: - Human peripheral blood CD4+ T cells treated with PHA
- RPMI8226 cells

Western Blot

1 µg/mL
Sample: Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR)
under non-reducing conditions only

Human GITR/TNFRSF18 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human GITR/TNFRSF18 Biotinylated Antibody (Catalog # BAF689)
  • Standard: Recombinant Human GITR/TNFRSF18 Fc Chimera Protein, CF (Catalog # 689-GR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB689 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is
up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

References

  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Alternate Names

AITR, CD357, TNFRSF18

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Gene Symbol

TNFRSF18

UniProt

Q9Y5U5

Additional GITR/TNFRSF18 Products

Product Documents for Human GITR/TNFRSF18 Antibody (110416)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GITR/TNFRSF18 Antibody (110416)

For research use only

Citations for Human GITR/TNFRSF18 Antibody (110416)

Customer Reviews for Human GITR/TNFRSF18 Antibody (110416) (1)

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  • Human GITR/TNFRSF18 Antibody
    Name: Paul Fisher
    Application: Flow Cytometry
    Sample Tested: Transfected cells
    Species: Human
    Verified Customer | Posted 06/29/2016
    looked for expression of GITR on a tranfected overexpressing cell line and it worked well versus an isotype control by flow cytometry

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