Human Hexim 1 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF8106N Datasheet / COA / SDS

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AF8106N-100UG

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Human Hexim 1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hexim 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Hexim 2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Hexim 1
His213-Asp359
Accession # O94992

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Hexim 1

Hexim 1, also known as CLP1, EDG1, HIS1, and MAQ1, is an approximately 60 kDa transcriptional regulator that binds double stranded RNA and inhibits RNA polymerase II. Hexim 1 plays an important role in many processes including satellite muscle cell regeneration, cardiovascular development, and HIV-1 replication. It can form homodimers and heterodimers with Hexim 2, and its activity is regulated by phosphoylation at Ser158. Hexim 1 inhibits the kinase activity of the elongation factor P-TEFb. This inhibition can be relieved by Hexim 1 interaction with Nucleophosphmin or by cellular exposure to hexamtehylene bisacetamide. Hexim 1 inhibits the transactivation function of the glucocorticoid, estrogen, and androgen receptors. It can inhibit the cellular response to hypoxia and promote cancer progression or, alternatively, can inhibit VEGF mediated angiogenesis and suppress metastasis. Within aa 213-359, human Hexim 1 shares 95% and 94% aa sequence identity with mouse and rat Hexim 1, respectively.

Long Name

Hexamethylene Bis-Acetamide Inducible 1

Entrez Gene IDs

10614 (Human); 192231 (Mouse); 498008 (Rat)

Alternate Names

Cardiac lineage protein 1; CLP1; CLP-1; CLP1hexamethylene-bis-acetamide-inducible transcript 1; EDG1HMBA-inducible; Estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide inducible 1; Hexamethylene bis-acetamide-inducible protein 1; hexamthylene bis-acetamide inducible 1; Hexim 1; HEXIM1; HIS1; HIS1FLJ13562; HMBA-inducible; MAQ1; MAQ1hexamethylene bisacetamide-inducible protein; menage a quatre 1; Menage a quatre protein 1; protein HEXIM1