Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Development (Capture), ELISA Microarray Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24516
View all HGF Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)

Specificity

Detects recombinant human (rh)HGF in direct ELISAs and Western blots. In direct ELISAs, less than 0.25% cross-reactivity was observed with rhMSP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human HGF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human HGF (Catalog # 294-HG)
under non-reducing conditions only

Human HGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human HGF Biotinylated Antibody (Catalog # BAF294)
  • Standard: Recombinant Human HGF Protein (Catalog # 294-HG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB694 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HGF

HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

Additional HGF Products

Product Documents for Human HGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HGF Antibody

For research use only

Citations for Human HGF Antibody

Customer Reviews for Human HGF Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human HGF Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 的 2 reviews Showing All
Filter By:
Clear Filters X
  • Human HGF Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver myofibroblasts
    Species: Human
    Verified Customer | Posted 10/11/2021
    Human HGF Antibody MAB694
  • Human HGF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/16/2017
    A sandwich ELISA was made with MAB694 as the capture and BAF294 as the detection using 294-HG as the immunoassay calibrator.
    Human HGF Antibody MAB694

There are no reviews that match your criteria.

Showing  1 - 2 的 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies