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Human HIF-2 alpha /EPAS1 Antibody

Catalog #: MAB2886
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MAB2886
MAB2886-SP

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Detection of Human HIF‑2 alpha /EPAS1 by Western Blot.
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Human HIF-2 alpha /EPAS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HIF-2 alpha /EPAS1 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 318404
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIF-2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HIF-2a/EPAS1 antibody by Western Blot. View Larger

Detection of Human HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line (HIF-2a positive), Caki-2 human clear cell carcinoma epithelial cell line (HIF-2a negative), and HeLa human cervical epithelial carcinoma cell line (HIF-2a negative). PVDF Membrane was probed with 1 µg/mL of Human HIF-2a/EPAS1 Monoclonal Antibody (Catalog # MAB2886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-2a/EPAS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha

Long Name
Hypoxia-inducible Transcription Factor 2 alpha
Entrez Gene IDs
2034 (Human); 13819 (Mouse)
Alternate Names
Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

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Citation for Human HIF-2 alpha /EPAS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
    Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
    J. Clin. Invest., 2010-02-08;120(3):827-39.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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