Human HLA-E Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB11547R Datasheet / COA / SDS

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FAB11547R-100UG

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All HLA-E Antibodies

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Human HLA-E Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide around aa 80 in Direct ELISA

Source

Monoclonal Mouse IgG2a Clone # 1079755

Purification

Protein A or G purified

Immunogen

HLA-E containing synthetic peptide
Accession # P13747

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HLA-E

HLA-E is a class I histocompatibility antigen involved in immune self-nonself discrimination. HLA-E classically binds epitopes from HLA-A, HLA-B, HLA-C and HLA-G signal peptides and serves as a ligand for CD94/NKG2A and CD94/NKG2C receptors on NK and t-cell subsets. HLA-E down-regulates NK cell cytolytic activity when presented on the cell surface. It can also restrict non-canonical CD8+ T cells during natural infection with pathogens. HLA-E protein expression in normal human nonlymphoid organs is restricted to endothelial cells. It is also basally expressed by B and T-lymphocytes, NK cells and macrophages. TNF-alpha, IL-1beta and IFN-gamma up-regulated cell-surface expression of HLA-EE.

Long Name

Major Histocompatibility Complex, Class I, E

Entrez Gene IDs

3133 (Human)

Alternate Names

DKFZp686P19218; EA1.2; EA2.1; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; HLA-6.2; HLAE; HLA-E; lymphocyte antigen; major histocompatibility complex, class I, E; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1