Human HVEM/TNFRSF14 Antibody (94801)

R&D Systems | Catalog # MAB356

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 94801
View all HVEM/TNFRSF14 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
Pro37-Val202 (Ser108Thr and Ala140Arg)
Accession # AAB58354

Specificity

Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human HVEM/TNFRSF14 Antibody (94801)

Detection of HVEM/TNFRSF14 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB356, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Human HVEM/TNFRSF14 by Block/Neutralize

Detection of Human HVEM/TNFRSF14 by Block/Neutralize

Differential inhibition of HVEM/CD160 binding with benchmark tool antibodies. TRF assay measuring the potency of different antibodies to inhibit the binding of recombinant human HVEM-Fc chimera to CD160+ CHO-K1 cells. A) CD160 monoclonal antibodies inhibit binding of HVEM-Fc to both CD160-GPI and CD160-TM isoforms. B) Polyclonal HVEM (left panel) and monoclonal HVEM (right panel) antibodies both enhance binding of HVEM-Fc to CD160-TM isoform. The polyclonal anti-HVEM inhibits HVEM-Fc binding to CD160-GPI (left panel). Antibody concentrations are plotted on the X axis whereas, the calculated percentage of inhibition of binding is plotted on the Y axis. Matched isotype control antibody for each individual antibody candidate was also used in the assay (empty circles and squares). CTL = control, mAb = monoclonal antibody, pAb = polyclonal antibody. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25179432), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human HVEM / TNFRSF14 ELISA Standard Curve

Human HVEM / TNFRSF14 ELISA Standard Curve

Human HVEM/TNFRSF14 was serially diluted and captured by Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB356) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human HVEM/TNFRSF14 Antibody (94801)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood lymphocytes

Western Blot

1 µg/mL
Sample: Recombinant Human HVEM/TNFRSF14 Fc Chimera (Catalog # 356-HV)

Human HVEM/TNFRSF14 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human HVEM/TNFRSF14 Biotinylated Antibody (Catalog # BAF356)
  • Standard: Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 356-HV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB356 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta  (lymphotoxin  alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

References

  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Mauri, D.N. et al. (1998) Immunity 8:21.
  3. Montgomery, R.I. et al. (1996) Cell 87:427.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Gene Symbol

TNFRSF14

UniProt

AAB58354

Additional HVEM/TNFRSF14 Products

Product Documents for Human HVEM/TNFRSF14 Antibody (94801)

Certificate of Analysis

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Product Specific Notices for Human HVEM/TNFRSF14 Antibody (94801)

For research use only

Citations for Human HVEM/TNFRSF14 Antibody (94801)

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  • Human HVEM/TNFRSF14 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: B lymphocytes
    Species: Human
    Verified Customer | Posted 11/09/2021
    B lymphocytes, Human HVEM/TNFRSF14 Antibody
    Human HVEM/TNFRSF14 Antibody (94801) MAB356

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