Human ICAM-3/CD50 Antibody

R&D Systems | Catalog # AF813

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ICAM-3/CD50 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473

Specificity

Detects human ICAM‑3/CD50 in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human ICAM-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ICAM-3/CD50 Antibody

Human ICAM-3 / CD50 ELISA Standard Curve

Human ICAM-3 / CD50 ELISA Standard Curve

Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC) was serially diluted and captured by Mouse Anti-Human ICAM‑3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM‑3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human ICAM-3/CD50 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Entrez Gene IDs

3385 (Human)

Gene Symbol

ICAM3

UniProt

CAA49473

Additional ICAM-3/CD50 Products

Product Documents for Human ICAM-3/CD50 Antibody

Certificate of Analysis

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Product Specific Notices for Human ICAM-3/CD50 Antibody

For research use only

Citations for Human ICAM-3/CD50 Antibody

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