Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.
Human IFN-alpha/beta R1 Antibody (1056928)
R&D Systems | Catalog # MAB2451
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1056928
Loading...
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730
Lys28-Lys436
Accession # AAA52730
Specificity
Detects human IFN-alpha / beta R1 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IFN-alpha/beta R1 Antibody (1056928)
Detection of Human IFN‑ alpha / beta R1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IFN‑ alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of IFN‑ alpha / beta R1 in U937 human histiocytic lymphoma cell line by Flow Cytometry.
U937 human histiocytic lymphoma cells were stained with Mouse Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2451, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human IFN-alpha/beta R1 Antibody (1056928)
Application
Recommended Usage
Flow Cytometry
25 µg/mL
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line.
Sample: HeLa human cervical epithelial carcinoma cell line.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IFN-alpha/beta R1
Long Name
Interferon alpha/beta Receptor 1
Alternate Names
IFN-aR1, IFN-bR1, IFNAR1, IFNbR1
Gene Symbol
IFNAR1
UniProt
Additional IFN-alpha/beta R1 Products
Product Documents for Human IFN-alpha/beta R1 Antibody (1056928)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN-alpha/beta R1 Antibody (1056928)
For research use only
Related Research Areas
Customer Reviews for Human IFN-alpha/beta R1 Antibody (1056928)
There are currently no reviews for this product. Be the first to review Human IFN-alpha/beta R1 Antibody (1056928) and earn rewards!
Have you used Human IFN-alpha/beta R1 Antibody (1056928)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
