Human IL-11 Antibody

Catalog #: MAB218R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB218R-100
MAB218R-SP

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Cell Proliferation Induced by IL-11 and Neutralization by Human IL-11 Antibody.

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All IL-11 Antibodies

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Human IL-11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-11 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2A Clone # 22626R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human IL‑11
Pro22-Leu199
Accession # P20809

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑11 (Catalog # 218-IL)

Neutralization

Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND₅₀) is typically5-15 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL-11 and Neutralization by Human IL-11 Antibody. Recombinant Human IL-11 (218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-11 Monoclonal Antibody (Catalog # MAB218R). The ND₅₀ is typically 5-15 µg/mL

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11

Interleukin-11 (IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric receptor complex consisting of the IL-11 Ra chain and the gp130 chain shared by the receptors for the IL-6 cytokine family. IL-11 has multiple effects on hematopoietic cells and osteoblasts.

Long Name

Interleukin 11

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Alternate Names

Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin